Frank Witzmann, Ph.D.
Full Member of Graduate Faculty
Department of Cellular & Integrative Physiology
Indiana University School of Medicine
635 Barnhill Drive, Room 362A
Indianapolis, Indiana 46202-2111
E-mail: fwitzman @ iupui.edu
Education / Training
1976, B.A., Defiance College, Defiance, Ohio
1978, M.S. in Biology (Human Bioenergetics), Ball State University, Muncie, Indiana
1981, Ph.D. in Biology (Human Physiology), Marquette University, Milwaukee, Wisconsin
My research involves the application of proteomic techniques to the detection and analysis of protein expression in a variety of research paradigms. Specific projects include 1) the analysis of differential protein expression by cells and tissues exposed to Engineered Nanomaterials (ENM) in vivo or in vitro, 2) the study of alcohol effects on protein expression in various brain cell types and regions, 3) the characterization of serum proteins associated with alcohol abuse, and 4) assessment of altered protein expression in Polycystic Kidney Disease (PKD) and Acute kidney Injury (AKI).
In conducting these studies, we apply complementary proteomic technologies to address the unique analytical demands associated with each project. We use solution isoelectric focusing, large format one- and two-dimensional gel electrophoretic separations and image analysis coupled to tandem mass spectrometry (LC-MS/MS) to analyze and identify differential protein expression.
As a gel-free alternative, we have developed a label-free, quantitative mass spectrometric platform that exploits both single- and multi-dimensional liquid chromatographic approaches and delivers a global analysis of differential protein expression in complex mixtures. Using both statistical, bioinformatic, and pathway analysis strategies, alterations in either the quantitative expression or post-translational modification of individual proteins or altered protein expression “patterns” from tissues, cells and cell fractions detected by these approaches is assessed. Protein expression information obtained in this way can be used as indicators or “molecular biomarkers” of primary or secondary cellular effects, or used to better understand the molecular mechanisms, as represented by the altered proteome, that trigger altered or impaired physiological function, cellular injury, and disease.
National Institutes of Health, “Proximal Tubule Albumin Transport in Disease States” Co-investigator, 10/01/11 – 09/30/16, RO1DK091623 (Molitoris)
National Institutes of Health, “Nanoparticle Effects on Epithelial Cell Protein Expression and Function” Frank A. Witzmann, Principal Investigator. 06/01/2008 – 04/30/2013. R01GM085218-01