Create an environment fostering outstanding research and teaching by our commitment to obtaining and imparting knowledge of molecular, cellular, and integrative physiology.
Intravital microscopy (IVM) measurements of the response of the protein kinase A (PKA) biosensor AKAR4.1 to glucagon in hepatocytes in the intact mouse liver. The PKA biosensor was introduced into mice by tail vein injection of an adenoviral vector, resulting in extensive expression of AKAR4.1 in the liver after 7 days. Mice were fasted for 3 hrs prior to intrapertoneal injection of glucagon (200ug/kg) followed by IVM. (A) The background-corrected Venus to Turquoise FP emission ratios were monitored from 10 individual cells (+SE) over 10 minutes demonstrating the robust activation of PKA in response to glucagon. (B) Ratio images from a single image plane in mouse liver at the indicated time points (scale bar is 10 um). Tao et al. (2015) Am J Physiol Cell Physiol. Sep 2
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